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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
28/03/2011 |
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Data da última atualização: |
29/04/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
OLIVEIRA, T. M.; PIROVANI, C. P.; COSTA, M. G. C.; SOARES FILHO, W. dos S.; GESTEIRA, A. S. |
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Afiliação: |
T . M. Oliveira, UESC; Carlos Priminho Pirovani, UESC; Marcio Gilberto Cardoso Costa, UESC; WALTER DOS SANTOS SOARES FILHO, CNPMF; ABELMON DA SILVA GESTEIRA, CNPMF. |
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Título: |
Identification of proteins involved in response mechanisms to water stress in citrus. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM. |
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Idioma: |
Inglês |
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Notas: |
pdf 34710 |
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Conteúdo: |
The citrus industry is an important economic sector worldwide, with Brazil being the world?s largest producer and exporter of orange juice frozen concentrated orange. In Brazil, among the abiotic stresses, drought is one that most often negative impact on productivity of citrus. Different families proteins are known to be associated with plant responses to stresses abiotic, again being synthesized, as well as having their concentrations accumulated or diminished. This proposal aims to identify key proteins that are related to responses to drought stress in tolerant genotypes of citrus and likely forced into this position. Therefore, two contrasting varieties of citrus, Rangpur lime and tangerine Sunki were subjected to two water regimes: (i) Control: plants grown in field capacity and (ii) water stress, plants grown without irrigation. The changes in protein dynamics in the leaves and roots were analyzed at three different times during water stress. Proteins were extracted using the method of ADP (Acetone Power Dry), followed by SDS-dense phenol. The quantification was done using the 2-D Quantification kit. Protein both treatments were extracted in triplicate and separated using SDS-PAGE bi-dimensional 12%. The analysis of 2D maps showed about 300 spots per gel, showing the differentially expressed proteins. The proteins were identified in the MS Q-TOF and were classified into several categories, including protein related to photosynthesis, proteins involved in metabolism and production of energy, proteins related to metabolism of carbohydrates and proteins unknown biological function. Identifying these proteins will help in understanding of the biochemical processes involved in the mechanisms of response to drought and provide input to the development of rootstocks drought tolerant, so use this information to improve this important group of plants through genetic manipulation. MenosThe citrus industry is an important economic sector worldwide, with Brazil being the world?s largest producer and exporter of orange juice frozen concentrated orange. In Brazil, among the abiotic stresses, drought is one that most often negative impact on productivity of citrus. Different families proteins are known to be associated with plant responses to stresses abiotic, again being synthesized, as well as having their concentrations accumulated or diminished. This proposal aims to identify key proteins that are related to responses to drought stress in tolerant genotypes of citrus and likely forced into this position. Therefore, two contrasting varieties of citrus, Rangpur lime and tangerine Sunki were subjected to two water regimes: (i) Control: plants grown in field capacity and (ii) water stress, plants grown without irrigation. The changes in protein dynamics in the leaves and roots were analyzed at three different times during water stress. Proteins were extracted using the method of ADP (Acetone Power Dry), followed by SDS-dense phenol. The quantification was done using the 2-D Quantification kit. Protein both treatments were extracted in triplicate and separated using SDS-PAGE bi-dimensional 12%. The analysis of 2D maps showed about 300 spots per gel, showing the differentially expressed proteins. The proteins were identified in the MS Q-TOF and were classified into several categories, including protein related to photosynthesis, proteins involved in metabolism an... Mostrar Tudo |
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Palavras-Chave: |
Differential expression; Drought stress; Proteomic; Rootstock. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02637nam a2200217 a 4500
001 1883133
005 2011-04-29
008 2011 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, T. M.
245 $aIdentification of proteins involved in response mechanisms to water stress in citrus.
260 $aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM.$c2011
500 $apdf 34710
520 $aThe citrus industry is an important economic sector worldwide, with Brazil being the world?s largest producer and exporter of orange juice frozen concentrated orange. In Brazil, among the abiotic stresses, drought is one that most often negative impact on productivity of citrus. Different families proteins are known to be associated with plant responses to stresses abiotic, again being synthesized, as well as having their concentrations accumulated or diminished. This proposal aims to identify key proteins that are related to responses to drought stress in tolerant genotypes of citrus and likely forced into this position. Therefore, two contrasting varieties of citrus, Rangpur lime and tangerine Sunki were subjected to two water regimes: (i) Control: plants grown in field capacity and (ii) water stress, plants grown without irrigation. The changes in protein dynamics in the leaves and roots were analyzed at three different times during water stress. Proteins were extracted using the method of ADP (Acetone Power Dry), followed by SDS-dense phenol. The quantification was done using the 2-D Quantification kit. Protein both treatments were extracted in triplicate and separated using SDS-PAGE bi-dimensional 12%. The analysis of 2D maps showed about 300 spots per gel, showing the differentially expressed proteins. The proteins were identified in the MS Q-TOF and were classified into several categories, including protein related to photosynthesis, proteins involved in metabolism and production of energy, proteins related to metabolism of carbohydrates and proteins unknown biological function. Identifying these proteins will help in understanding of the biochemical processes involved in the mechanisms of response to drought and provide input to the development of rootstocks drought tolerant, so use this information to improve this important group of plants through genetic manipulation.
653 $aDifferential expression
653 $aDrought stress
653 $aProteomic
653 $aRootstock
700 1 $aPIROVANI, C. P.
700 1 $aCOSTA, M. G. C.
700 1 $aSOARES FILHO, W. dos S.
700 1 $aGESTEIRA, A. S.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
21/10/2019 |
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Data da última atualização: |
21/10/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
OLIVEIRA, K. C. de; GUIMARÃES, P. de S.; BAZIOLI, J. M.; MARTINATI, J. C.; SANTOS, M. M. dos; PADILHA, L.; GUERREIRO-FILHO, O.; MALUF, M. P. |
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Afiliação: |
Kenia Carvalho de Oliveira, Coffee Center Agronomic Institute - IAC; Paula de Souza Guimarães, Coffee Center Agronomic Institute - IAC; Jaqueline Moraes Bazioli, Coffee Center Agronomic Institute - IAC; Juliana Camargo Martinati, Coffee Center Agronomic Institute - IAC; Mariana Martinis dos Santos, Coffee Center Agronomic Institute - IAC; LILIAN PADILHA, CNPCa; Oliveiro Guerreiro-Filho, Coffee Center Agronomic Institute - IAC; MIRIAN PEREZ MALUF, CNPCa. |
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Título: |
Effects of somatic embryogenesis on gene expression of cloned coffee heterozygous hybrids. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Acta Physiologiae Plantarum, v. 41, n. 7, July 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Cloning of superior coffee plants by somatic embryogenesis can assist breeding programs on reducing the cost and time for launch of new cultivars. This study aimed to evaluate the efficiency of this methodology for cloning coffee trees with high heterozygosity, and to gather evidence that clonal progenies are faithful copies of mother plants. Selected plants IAC1 and IAC 2 from Coffea arabica breeding populations, resistant to leaf rust and leaf miner, respectively, were cloned via indirect somatic embryogenesis. Expression of selected genes involved in biological processes potentially affected by in vitro cultivation was evaluated by quantitative analysis. Genes encoding proteins associated with maintenance of DNA integrity and control of cell cycle presented predictable expression patterns along the clonal multiplication process. There were differences in the expression pattern of genes linked to in vitro cultivation-related stress, which were observed comparing either IAC 1 and IAC2 genotypes or clones and their corresponding mother plant. Those analyses suggest that the somatic embryogenesis does not lead to major genomic instability and clones are identical copies of mother plants, even with detected differences in the expression of genes that influence the response of in vitro cultivation. |
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Palavras-Chave: |
Differential expression; In vitro cultivation. |
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Thesagro: |
Coffea Arábica. |
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Thesaurus NAL: |
Somaclonal variation. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/203223/1/Effects-of-somatic-embryogenesis-on-gene.pdf
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Marc: |
LEADER 02084naa a2200253 a 4500
001 2113261
005 2019-10-21
008 2019 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, K. C. de
245 $aEffects of somatic embryogenesis on gene expression of cloned coffee heterozygous hybrids.$h[electronic resource]
260 $c2019
520 $aCloning of superior coffee plants by somatic embryogenesis can assist breeding programs on reducing the cost and time for launch of new cultivars. This study aimed to evaluate the efficiency of this methodology for cloning coffee trees with high heterozygosity, and to gather evidence that clonal progenies are faithful copies of mother plants. Selected plants IAC1 and IAC 2 from Coffea arabica breeding populations, resistant to leaf rust and leaf miner, respectively, were cloned via indirect somatic embryogenesis. Expression of selected genes involved in biological processes potentially affected by in vitro cultivation was evaluated by quantitative analysis. Genes encoding proteins associated with maintenance of DNA integrity and control of cell cycle presented predictable expression patterns along the clonal multiplication process. There were differences in the expression pattern of genes linked to in vitro cultivation-related stress, which were observed comparing either IAC 1 and IAC2 genotypes or clones and their corresponding mother plant. Those analyses suggest that the somatic embryogenesis does not lead to major genomic instability and clones are identical copies of mother plants, even with detected differences in the expression of genes that influence the response of in vitro cultivation.
650 $aSomaclonal variation
650 $aCoffea Arábica
653 $aDifferential expression
653 $aIn vitro cultivation
700 1 $aGUIMARÃES, P. de S.
700 1 $aBAZIOLI, J. M.
700 1 $aMARTINATI, J. C.
700 1 $aSANTOS, M. M. dos
700 1 $aPADILHA, L.
700 1 $aGUERREIRO-FILHO, O.
700 1 $aMALUF, M. P.
773 $tActa Physiologiae Plantarum$gv. 41, n. 7, July 2019.
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